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Anti-LC3B Antibody (7R171)
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Catalog No. TMAB-08239
Anti-LC3B Antibody (7R171) is a Rabbit antibody targeting LC3B. Anti-LC3B Antibody (7R171) can be used in IF, IHC-Fr, IHC-P, WB.
Anti-LC3B Antibody (7R171)
Anti-LC3B Antibody (7R171)
Copy Product Info😃Good
Catalog No. TMAB-08239
Anti-LC3B Antibody (7R171) is a Rabbit antibody targeting LC3B. Anti-LC3B Antibody (7R171) can be used in IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $151 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $472 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-LC3B Antibody (7R171) is a Rabbit antibody targeting LC3B. Anti-LC3B Antibody (7R171) can be used in IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG/Kappa |
| Clone | 7R171 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human right occipital lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation LC3B Monoclonal Antibody, Unconjugated (TMAB-08239) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining. 11. 25 ug total protein per lane of various lysates (see on figure) probed with LC3B monoclonal antibody, unconjugated (TMAB-08239) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. |
| Application | |
| Recommended Dose | IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:1000-2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasmic. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3B binds to the autophagic membranes. |
| Tissue Specificity | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. LC3, a ubiquitin-like modifier protein, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. LC3 is expressed as 3 splice variants (LC3A, LC3B and LC3C), which exhibit different tissue distributions and are processed into cytosolic and autophagosomal membrane-bound forms, termed LC3-I and LC3-II, respectively. A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies, where LC3 is widely employed as a marker for autophagy. |
| Immunogen | A synthesized peptide: human LC3B |
| Antigen Species | Human |
| Gene Name | MAP1LC3B |
| Gene ID | |
| Protein Name | Microtubule-associated proteins 1A/1B light chain 3B |
| Uniprot ID | |
| Function | Probably involved in formation of autophagosomal vacuoles (autophagosomes). |
| Molecular Weight | Theoretical: 15 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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