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Anti-LATS2 Polyclonal Antibody
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Catalog No. TMAB-01051
Alias Warts like kinase, Serine/threonine protein kinase kpm, Serine/threonine kinase kpm, LATS large tumor suppressor homolog 2, LATS large tumor suppressor Drosophila homolog 2, Large tumor suppressor kinase 2, Large tumor suppressor homolog 2 Drosophila, Large tumor suppressor homolog 2, KPM, Kinase phosphorylated during mitosis protein, Kinase phosphorylated during mitosis, FLJ13161
Anti-LATS2 Polyclonal Antibody is a Rabbit antibody targeting LATS2. Anti-LATS2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-LATS2 Polyclonal Antibody
Anti-LATS2 Polyclonal Antibody
😃Good
Catalog No. TMAB-01051Alias Warts like kinase, Serine/threonine protein kinase kpm, Serine/threonine kinase kpm, LATS large tumor suppressor homolog 2, LATS large tumor suppressor Drosophila homolog 2, Large tumor suppressor kinase 2, Large tumor suppressor homolog 2 Drosophila, Large tumor suppressor homolog 2, KPM, Kinase phosphorylated during mitosis protein, Kinase phosphorylated during mitosis, FLJ13161
Anti-LATS2 Polyclonal Antibody is a Rabbit antibody targeting LATS2. Anti-LATS2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-LATS2 Polyclonal Antibody is a Rabbit antibody targeting LATS2. Anti-LATS2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Warts like kinase, Serine/threonine protein kinase kpm, Serine/threonine kinase kpm, LATS large tumor suppressor homolog 2, LATS large tumor suppressor Drosophila homolog 2, Large tumor suppressor kinase 2, Large tumor suppressor homolog 2 Drosophila, Large tumor suppressor homolog 2, KPM, Kinase phosphorylated during mitosis protein, Kinase phosphorylated during mitosis, FLJ13161 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Cow,Horse,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (LATS2) Polyclonal Antibody, Unconjugated (TMAB-01051) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 11. Sample: Lane 1: Mouse Heart Lysates Lane 2: Mouse Brain Lysates Lane 3: Mouse Stomach Lysates Lane 4: Mouse Testis Lysates Lane 5: Rat Heart Lysates Lane 6: Rat Brain Lysates Lane 7: Rat Stomach Lysates Lane 8: Human U2OS cell Lysates Lane 9: Human U251 cell Lysates Primary: Anti-LATS2 (TMAB-01051) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kDa Observed band size: 120 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human LATS2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Tumor suppressors,Kinases,Cytokinesis,Cdk Regulators |
| Molecular Weight | Theoretical: 120 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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