Anti-LAR Polyclonal Antibody is a Rabbit antibody targeting LAR. Anti-LAR Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Dog,Cow,Horse,Rabbit,Sheep)

1. Blank control (blue): Hela Cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-LAR/AF488 Conjugated antibody (TMAB-08219-AF488), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.
2. Blank control: MCF7.
Primary Antibody (green line): Rabbit Anti-LAR antibody (TMAB-08219)
Dilution: 2 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF488
Dilution: 1 μg /test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human PTPRF

Human

PTPRF

Receptor-type tyrosine-protein phosphatase F

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity.
The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.