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Anti-Laminin 5 Polyclonal Antibody

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Catalog No. TMAB-01043

Anti-Laminin 5 Polyclonal Antibody is a Rabbit antibody targeting Laminin 5. Anti-Laminin 5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-Laminin 5 Polyclonal Antibody

Anti-Laminin 5 Polyclonal Antibody

😃Good
Catalog No. TMAB-01043
Anti-Laminin 5 Polyclonal Antibody is a Rabbit antibody targeting Laminin 5. Anti-Laminin 5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF

Reactivity: Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit)
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated (TMAB-01043) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationSecreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundLaminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is an isoform composed of three distinct subunits, alpha 3, beta 3 and gamma 2, which are bound to each other in a cross-shaped molecule by disulphide bonds. It is a complex glycoprotein thought to be involved in cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes. It is also involved in signal transduction via tyrosine phosphorylation of pp125-FAK and p80, and differentiation of keratinocytes. The laminin alpha 3 subunit is also thought to be a component of laminin 6 and laminin 7
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human LAMA3
Antigen Species
Human
Gene ID
3909
Uniprot ID
Biology Area
Extracellular matrix,Extracellular Matrix,Intermediate Filaments,ECM Proteins,Basal Lamina
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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