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Anti-Laminin 5 Polyclonal Antibody is a Rabbit antibody targeting Laminin 5. Anti-Laminin 5 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF Reactivity: Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit) |
Ig Type | IgG |
Reactivity | Mouse (predicted:Human,Rat,Dog,Pig,Cow,Horse,Rabbit) |
Verified Activity | Tissue/cell: mouse stomach wall; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-Laminin 5 Polyclonal Antibody, Unconjugated (TMAB-01043) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
Application | IHC-P,IHC-Fr,IF |
Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | Laminins are basement membrane components thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminin 5 is an isoform composed of three distinct subunits, alpha 3, beta 3 and gamma 2, which are bound to each other in a cross-shaped molecule by disulphide bonds. It is a complex glycoprotein thought to be involved in cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes. It is also involved in signal transduction via tyrosine phosphorylation of pp125-FAK and p80, and differentiation of keratinocytes. The laminin alpha 3 subunit is also thought to be a component of laminin 6 and laminin 7 |
Immunogen | KLH conjugated synthetic peptide: human LAMA3 |
Antigen Species | Human |
Gene ID | 3909 |
Uniprot ID | |
Biology Area | Extracellular matrix,Extracellular Matrix,Intermediate Filaments,ECM Proteins,Basal Lamina |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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