Anti-lamin B1, Nuclear Loading Control Antibody (2E4) is a Mouse antibody targeting lamin B1, Nuclear Loading Control. Anti-lamin B1, Nuclear Loading Control Antibody (2E4) can be used in WB,IHC-P,IHC-Fr,IF.

IgG1

2E4

Human,Rat

1. Sample:
Lane 1: Rat Pancreas tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Rat Thymus tissue lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Molt-4 cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human U-2os cell ysates
Lane 8: Human THP-1 cell lysates
Primary: Anti-Lamin B (TMAB-08199) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
2. Sample:
Lane 1: Human Molt-4 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human U-2os cell ysates
Primary: Anti-Lamin B (TMAB-08199) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
8. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
9. Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
10. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
11. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
12. Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
13. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
14. Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (ascites of TMAB-08199) at 1:300 overnight at 4°C, followed by conjugation to the AffiniPure Goat Anti-Mouse IgG H&L-HRP and DAB staining.
15. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with lamin B1 Monoclonal Antibody, Unconjugated (TMAB-08199) at 1: 200 overnight at 4°C. Followed by conjugated Goat Anti-Mouse IgG antibody (green), DAPI (blue) was used to stain the cell nuclei.

WB: 1:1000-5000; IHC-P: 1:200-1000; IHC-Fr: 1:200-1000; IF: 1:200-1000

Monoclonal

Recombinant Protein: human Lamin B Protein

Human

LMNB1

Lamin-B1

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.