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Anti-LAMC1 Polyclonal Antibody
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Catalog No. TMAB-08190
Anti-LAMC1 Polyclonal Antibody is a Rabbit antibody targeting LAMC1. Anti-LAMC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-LAMC1 Polyclonal Antibody
Anti-LAMC1 Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08190
Anti-LAMC1 Polyclonal Antibody is a Rabbit antibody targeting LAMC1. Anti-LAMC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $220 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-LAMC1 Polyclonal Antibody is a Rabbit antibody targeting LAMC1. Anti-LAMC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Cow) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Laminin B2 gamma 1) Polyclonal Antibody, Unconjugated (TMAB-08190) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. 2. Sample: Lane 1: Rat Placenta tissue lysates Lane 2: Rat Spleen tissue lysates Primary: Anti-LAMC1 (TMAB-08190) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 174 kDa Observed band size: 230 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Tissue Specificity | Found in the basement membranes. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. The gamma 1 chain, formerly thought to be a beta chain, contains structural domains similar to beta chains, however, lacks the short alpha region separating domains I and II. The structural organization of this gene also suggested that it had diverged considerably from the beta chain genes. |
| Immunogen | KLH conjugated synthetic peptide: human LAMC1 |
| Antigen Species | Human |
| Gene Name | LAMC1 |
| Gene ID | |
| Protein Name | Laminin subunit gamma-1 |
| Uniprot ID | |
| Function | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. |
| Molecular Weight | Theoretical: 174 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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