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Anti-LAG3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01042
Alias lymphocyte-activation gene 3, LAG-3, CD223

Anti-LAG3 Polyclonal Antibody is a Rabbit antibody targeting LAG3. Anti-LAG3 Polyclonal Antibody can be used in FCM.

Anti-LAG3 Polyclonal Antibody

Anti-LAG3 Polyclonal Antibody

😃Good
Catalog No. TMAB-01042Alias lymphocyte-activation gene 3, LAG-3, CD223
Anti-LAG3 Polyclonal Antibody is a Rabbit antibody targeting LAG3. Anti-LAG3 Polyclonal Antibody can be used in FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-LAG3 Polyclonal Antibody is a Rabbit antibody targeting LAG3. Anti-LAG3 Polyclonal Antibody can be used in FCM.
Aliaslymphocyte-activation gene 3, LAG-3, CD223
Ig Type
IgG
Reactivity
Mouse (predicted:Human,Rat,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Blank control: Ctll-2. Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (TMAB-01042)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control: Ctll-2. Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (TMAB-01042)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
FCM
Recommended Dose
FCM: 1μg /test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundLymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human LAG-3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Regulatory T Cells,CD,NK Cells,Other secreted molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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