Anti-LAG3 Polyclonal Antibody is a Rabbit antibody targeting LAG3. Anti-LAG3 Polyclonal Antibody can be used in FCM.

IgG

Mouse (predicted:Human,Rat,Pig,Cow,Horse,Rabbit)

1. Blank control: Ctll-2. Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (TMAB-01042)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Blank control: Ctll-2. Primary Antibody (green line): Rabbit Anti-LAG-3 antibody (TMAB-01042)
Dilution: 1 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human LAG-3

Human

Regulatory T Cells,CD,NK Cells,Other secreted molecules