Anti-Lactoferrin Antibody (4V384) is a Mouse antibody targeting Lactoferrin. Anti-Lactoferrin Antibody (4V384) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

4V384

Human

1. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Lactoferrin) Monoclonal Antibody, Unconjugated (TMAB-08184) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.
2. Sample:
Lane 1: Human Lactoferrin
Primary: Anti-Lactoferrin (TMAB-08184) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:400-800; IF: 1:100-500

Monoclonal

Purified native Human Lactoferrin protein

Human

LTF

Lactotransferrin

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Isoform DeltaLf: transcription factor with antiproliferative properties and inducing cell cycle arrest. Binds to DeltaLf response element found in the SKP1, BAX, DCPS, and SELH promoters.extracellular cation concentration.