Anti-L-selectin Polyclonal Antibody is a Rabbit antibody targeting L-selectin. Anti-L-selectin Polyclonal Antibody can be used in FCM,IHC-Fr,WB.

IgG

Mouse,Rat (predicted:Cow,Rabbit)

1. Blank control: mouse spleen cells (blue). Primary Antibody: Rabbit Anti-CD62L antibody (TMAB-01084), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (TMAB-01084, 1 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice.
2. Sample:
LympHnode (Mouse) Lysate at 40 μg
LympHnode (Rat) Lysate at 40 μg
Primary: Anti-CD62L (TMAB-01084) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 43 kDa

WB: 1:500-2000; IHC-Fr: 1:100-500; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: mouse CD62L

Mouse

SELL

L-selectin

Blood,Regulatory T Cells,CD,T Lymphocytic Lineage,Neutrophil Lineage

Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.