Anti-kynureninase/KYNU Antibody (7U502) is a Rabbit antibody targeting kynureninase/KYNU. Anti-kynureninase/KYNU Antibody (7U502) can be used in WB,ICC/IF,IP.

Monoclonal Rabbit IgG

7U502

Human

1. Anti-KYNU rabbit monoclonal antibody at 1:500 dilution.

-Lane A: A549 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:52 kDa.

-Observed band size:52 kDa.

2. KYNU-HIS was immunoprecipitated using:

-Lane A:0.5 mg A549 Whole Cell Lysate

-0.5 µL anti-KYNU-HIS rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-KYNU-HIS rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution.

-Developed using the DAB staining technique.

-Performed under reducing conditions.

-Predicted band size: 52 kDa.

-Observed band size: 52 kDa.

3. Immunofluorescence staining of KYNU in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KYNU monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

WB,ICC/IF,IP

WB: 1:500-1:1000; ICC-IF: 1:20-1:100; IP: 0.2-1 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Human KYNU Protein (TMPY-02479)

Human