Anti-Kv1.8 Polyclonal Antibody is a Rabbit antibody targeting Kv1.8. Anti-Kv1.8 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Rabbit)

1. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-Kv1.8 (TMAB-08174) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
2. Sample: Stomach (Mouse) Lysate at 40 μg
Primary: Anti-Kv1.8 (TMAB-08174) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Kv1.8 Polyclonal Antibody, Unconjugated (TMAB-08174) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
4. Protein:
stomach(mouse) lysates at 40 μg;
kidney (mouse) lysates at 40 μg;
Primary: Anti-Kv1.8 (TMAB-08174) at 1:300;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000;
ECL excitated the fluorescence;
Predicted band size: 58 kD
Observed band size: 58 kD
5. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-Kv1.8 Polyclonal Antibody, Unconjugated (TMAB-08174) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Kv1.8

Human

KCNA10

Potassium voltage-gated channel subfamily A member 10

Mediates voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. The channel activity is up-regulated by cAMP.