Anti-Kv1.2 Polyclonal Antibody is a Rabbit antibody targeting Kv1.2. Anti-Kv1.2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse (predicted:Human,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep)

1. Sample: Spinal cord (Mouse) Lysate at 40 μg
Primary: Anti-Kv1.2 (TMAB-08172) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
2. Sample: Cerebellum (Mouse) Lysate at 40 μg
Primary: Anti-Kv1.2 (TMAB-08172) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Kv1.2) Polyclonal Antibody, Unconjugated (TMAB-08172) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Kv1.2) Polyclonal Antibody, Unconjugated (TMAB-08172) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human Kv1.2

Human

KCNA2

Potassium voltage-gated channel subfamily A member 2

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.