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Anti-KRT7 Antibody (5N725) is a Mouse antibody targeting KRT7. Anti-KRT7 Antibody (5N725) can be used in WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $198 | 7-10 days | 7-10 days |
| Description | Anti-KRT7 Antibody (5N725) is a Mouse antibody targeting KRT7. Anti-KRT7 Antibody (5N725) can be used in WB. |
| Ig Type | Mouse IgG2a |
| Clone | 5N725 |
| Reactivity | Human |
| Specificity | Human Cytokeratin 7/KRT7 |
| Verified Activity | Anti-KRT7 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:51 kDa. -Observed band size:51 kDa |
| Application | |
| Recommended Dose | WB: 1:500-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the Human Cytokeratin 7/KRT7. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | KRT7 (Keratin 7) is a Protein Coding gene. The protein encoded by this gene is a member of the keratin gene family. One AS, designated Homo sapiens keratin 7 (KRT7-AS), was selected due to its marked upregulation and concordant expression with its cognate sense counterpart, KRT7, in GC tissues and cell lines. KRT7-AS formed an RNA-RNA hybrid with KRT7 and controlled KRT7 expression at both the mRNA and the post-transcriptional levels. Moreover, forced overexpression of the KRT7-overlapping region (OL) of KRT7-AS (but not its non-KRT7-OL portions) increased keratin 7 protein levels in cells. Finally, forced overexpression of full-length KRT7-AS or OL KRT7-AS (but not its non-KRT7-OL regions) promoted GC cell proliferation and migration. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: center region of the Human Cytokeratin 7/KRT7 |
| Antigen Species | Human |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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