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Anti-KRT5 Antibody (6A459)
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Catalog No. TMAY-00123
Alias KRT5A, K5, EBS2F, EBS2E, EBS2D, EBS2C, EBS2B, EBS2A, EBS2, EBS1, DDD1, DDD, CK-5, CK5
Anti-KRT5 Antibody (6A459) is a Rabbit antibody targeting KRT5. Anti-KRT5 Antibody (6A459) can be used in WB,ICC/IF.
Anti-KRT5 Antibody (6A459)
Anti-KRT5 Antibody (6A459)
😃Good
Catalog No. TMAY-00123Alias KRT5A, K5, EBS2F, EBS2E, EBS2D, EBS2C, EBS2B, EBS2A, EBS2, EBS1, DDD1, DDD, CK-5, CK5
Anti-KRT5 Antibody (6A459) is a Rabbit antibody targeting KRT5. Anti-KRT5 Antibody (6A459) can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-KRT5 Antibody (6A459) is a Rabbit antibody targeting KRT5. Anti-KRT5 Antibody (6A459) can be used in WB,ICC/IF. |
| Alias | KRT5A, K5, EBS2F, EBS2E, EBS2D, EBS2C, EBS2B, EBS2A, EBS2, EBS1, DDD1, DDD, CK-5, CK5 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6A459 |
| Reactivity | Human |
| Verified Activity | 1. Anti-KRT5 rabbit monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:62 kDa. -Observed band size:62 kDa(We are unsure as to the identity of these extra bands.) 2. Immunofluorescence staining of KRT5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KRT5 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm . |
| Application | WB,ICC/IF |
| Recommended Dose | WB: 1:500-1:2000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Cytokeratin 5/KRT5. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Keratin 5 is the major type II keratin of the basal cells of the epidermis and other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide corresponding to the center region of the Human Cytokeratin 5/KRT5 |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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