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Anti-KRT5 Antibody (5H465)
😃Good
Catalog No. TMAY-00122
Alias KRT5A, K5, EBS2F, EBS2E, EBS2D, EBS2C, EBS2B, EBS2A, EBS2, EBS1, DDD1, DDD, CK-5, CK5
Anti-KRT5 Antibody (5H465) is a Mouse antibody targeting KRT5. Anti-KRT5 Antibody (5H465) can be used in WB.
Anti-KRT5 Antibody (5H465)
Anti-KRT5 Antibody (5H465)
😃Good
Catalog No. TMAY-00122Alias KRT5A, K5, EBS2F, EBS2E, EBS2D, EBS2C, EBS2B, EBS2A, EBS2, EBS1, DDD1, DDD, CK-5, CK5
Anti-KRT5 Antibody (5H465) is a Mouse antibody targeting KRT5. Anti-KRT5 Antibody (5H465) can be used in WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB Reactivity: Human |
| Alias | KRT5A, K5, EBS2F, EBS2E, EBS2D, EBS2C, EBS2B, EBS2A, EBS2, EBS1, DDD1, DDD, CK-5, CK5 |
| Ig Type | Mouse IgG1 |
| Clone | 5H465 |
| Reactivity | Human |
| Verified Activity | Anti-KRT5 mouse monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:62 kDa. -Observed band size:62 kDa |
| Application | WB |
| Recommended Dose | WB: 1:500-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human KRT5. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Keratin 5 is the major type II keratin of the basal cells of the epidermis and other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: center region of the human KRT5 |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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