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Anti-KRT20 Antibody (9H184)
😃Good
Catalog No. TMAB-01040
Anti-KRT20 Antibody (9H184) is a Rabbit antibody targeting KRT20. Anti-KRT20 Antibody (9H184) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Anti-KRT20 Antibody (9H184)
Anti-KRT20 Antibody (9H184)
😃Good
Catalog No. TMAB-01040
Anti-KRT20 Antibody (9H184) is a Rabbit antibody targeting KRT20. Anti-KRT20 Antibody (9H184) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μL | $153 | 7-10 days | |
| 50 μL | $273 | 7-10 days | |
| 100 μL | $488 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM Reactivity: Human,Rat |
| Ig Type | IgG |
| Clone | 9H184 |
| Reactivity | Human,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK20) Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK20) Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK20) Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK20 Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK20 Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK20 Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK20 Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK20 Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. 25 μg total protein per Lane of various lysates probed with CK20 monoclonal antibody, unconjugated (TMAB-01040) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 10. 4% Paraformaldehyde-fixed HT-29 (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (CK20) monoclonal Antibody, unconjugated (TMAB-01040) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green ) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control. 11. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CK20 Monoclonal Antibody, Unconjugated (TMAB-01040) at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (green), DAPI (blue) was used to stain the cell nucleus. |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20. |
| Immunogen | A synthesized peptide: human Cytokeratin 20 |
| Antigen Species | Human |
| Gene ID | 54474 |
| Uniprot ID | |
| Biology Area | Tumor biomarkers,Cytokeratins |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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