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Anti-KRT19 Antibody (4B372)
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Catalog No. TMAC-01084
Alias type I cytoskeletal 19, KRT19, Keratin-19 (K19), Keratin, type I cytoskeletal 19, Cytokeratin-19 (CK-19)
Anti-KRT19 Antibody (4B372) is a Rabbit antibody targeting KRT19. Anti-KRT19 Antibody (4B372) can be used in FCM,ICC/IF,IHC,WB.
Anti-KRT19 Antibody (4B372)
Anti-KRT19 Antibody (4B372)
😃Good
Catalog No. TMAC-01084Alias type I cytoskeletal 19, KRT19, Keratin-19 (K19), Keratin, type I cytoskeletal 19, Cytokeratin-19 (CK-19)
Anti-KRT19 Antibody (4B372) is a Rabbit antibody targeting KRT19. Anti-KRT19 Antibody (4B372) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $497 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-KRT19 Antibody (4B372) is a Rabbit antibody targeting KRT19. Anti-KRT19 Antibody (4B372) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | type I cytoskeletal 19, KRT19, Keratin-19 (K19), Keratin, type I cytoskeletal 19, Cytokeratin-19 (CK-19) |
| Ig Type | IgG |
| Clone | 4B372 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of Cytokeratin 19 on MCF-7 cell lysates using anti- Cytokeratin 19 antibody at 1/10,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cytokeratin 19 antibody. Counter stained with hematoxylin. 6. ICC staining Cytokeratin 19 in Ags cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. Flow cytometric analysis of MCF-7 cells with Cytokeratin 19 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:5000-10000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:10-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. For example, many types of cancer cells express Cytokeratin 19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous epithelium. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma. Cytokeratin 19 fragment levels in serum have been documented as a marker for lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a fragment of Cytokeratin 19, may be among the most useful tumor markers. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 40 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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