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Anti-KRT18 Polyclonal Antibody

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Catalog No. TMAB-01036
Alias type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein

Anti-KRT18 Polyclonal Antibody is a Rabbit antibody targeting KRT18. Anti-KRT18 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-KRT18 Polyclonal Antibody

Anti-KRT18 Polyclonal Antibody

😃Good
Catalog No. TMAB-01036Alias type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein
Anti-KRT18 Polyclonal Antibody is a Rabbit antibody targeting KRT18. Anti-KRT18 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-KRT18 Polyclonal Antibody is a Rabbit antibody targeting KRT18. Anti-KRT18 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Aliastype I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Chicken,Dog,Rabbit)
Verified Activity
1. Blank control (blue line): Hep G2 (blue).
Primary Antibody (green line): Rabbit Anti-CK18 antibody (TMAB-01036)
Dilution: 0.2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4°C) and then permeabilized with 90% methanol for 20 min at-20°C. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK18) Polyclonal Antibody, Unconjugated (TMAB-01036) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CK18) Polyclonal Antibody, Unconjugated (TMAB-01036) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-CK18 antibody (TMAB-01036)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: HepG2 (Human) Cell Lysate at 30 μg
Lane 3: A549 (Human) Cell Lysate at 30 μg
Lane 4: MCF-7 (Human) Cell Lysate at 30 μg
Primary: Anti-CK18 (TMAB-01036) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa
6. Tissue/cell: A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CK18) polyclonal Antibody, Unconjugated (TMAB-01036) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
7. Sample:
Hela KO CK18 (Human) Cell Lysate at 30 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-CK18 (TMAB-01036) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Application
WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 0.2μg /test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, perinuclear region. Nucleus, nucleolus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundKRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CK18
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Cytokeratins,Tumor Associated
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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