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Anti-KRT18 Antibody (5J111)
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Catalog No. TMAC-01083
Alias type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein
Anti-KRT18 Antibody (5J111) is a Rabbit antibody targeting KRT18. Anti-KRT18 Antibody (5J111) can be used in FCM,ICC/IF,IHC,WB.
Anti-KRT18 Antibody (5J111)
Anti-KRT18 Antibody (5J111)
😃Good
Catalog No. TMAC-01083Alias type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein
Anti-KRT18 Antibody (5J111) is a Rabbit antibody targeting KRT18. Anti-KRT18 Antibody (5J111) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $496 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-KRT18 Antibody (5J111) is a Rabbit antibody targeting KRT18. Anti-KRT18 Antibody (5J111) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | type I cytoskeletal 18, KRT18, Keratin-18 (K18), Keratin, type I cytoskeletal 18, Cytokeratin-18 (CK-18), CYK18, Cell proliferation-inducing gene 46 protein |
| Ig Type | IgG |
| Clone | 5J111 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of Cytokeratin 18 on different lysates using anti-Cytokeratin 18 antibody at 1/20,000 dilution. Positive control: Lane 1: A431, Lane 2: Mouse colon, Lane 3: Mouse kidney. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cytokeratin 18 antibody. Counter stained with hematoxylin. 7. ICC staining Cytokeratin 18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining Cytokeratin 18 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining Cytokeratin 18 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 10. Flow cytometric analysis of MCF-7 cells with Cytokeratin 18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:10000-50000; IHC: 1:50-200; ICC/IF: 1:100-500; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. For example, Cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while Cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas. Cytokeratin 18 contains two major phosphorylation sites on Ser 33 and Ser 52. Phosphorylation of Ser 18 is essential for the association of Cytokeratin 18 with 14-3-3 proteins and is involved in keratin organization and distribution. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 48 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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