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Anti-KRT16 Antibody (3O311)
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Catalog No. TMAC-01078
Alias type I cytoskeletal 16, KRT16A, KRT16, Keratin-16 (K16), Keratin, type I cytoskeletal 16, Cytokeratin-16 (CK-16)
Anti-KRT16 Antibody (3O311) is a Rabbit antibody targeting KRT16. Anti-KRT16 Antibody (3O311) can be used in FCM,ICC/IF,IHC,WB.
Anti-KRT16 Antibody (3O311)
Anti-KRT16 Antibody (3O311)
😃Good
Catalog No. TMAC-01078Alias type I cytoskeletal 16, KRT16A, KRT16, Keratin-16 (K16), Keratin, type I cytoskeletal 16, Cytokeratin-16 (CK-16)
Anti-KRT16 Antibody (3O311) is a Rabbit antibody targeting KRT16. Anti-KRT16 Antibody (3O311) can be used in FCM,ICC/IF,IHC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-KRT16 Antibody (3O311) is a Rabbit antibody targeting KRT16. Anti-KRT16 Antibody (3O311) can be used in FCM,ICC/IF,IHC,WB. |
| Synonyms | type I cytoskeletal 16, KRT16A, KRT16, Keratin-16 (K16), Keratin, type I cytoskeletal 16, Cytokeratin-16 (CK-16) |
| Ig Type | IgG |
| Clone | 3O311 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1,000 dilution. 2. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin. 5. ICC staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 7. ICC staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. Flow cytometric analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN). |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 51 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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