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Anti-KMT5A Polyclonal Antibody
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Catalog No. TMAB-08142
Anti-KMT5A Polyclonal Antibody is a Rabbit antibody targeting KMT5A. Anti-KMT5A Polyclonal Antibody can be used in WB,FCM.
Anti-KMT5A Polyclonal Antibody
Anti-KMT5A Polyclonal Antibody
Copy Product Info😃Good
Catalog No. TMAB-08142
Anti-KMT5A Polyclonal Antibody is a Rabbit antibody targeting KMT5A. Anti-KMT5A Polyclonal Antibody can be used in WB,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $529 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Bioactivity
Antigen Details
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| Description | Anti-KMT5A Polyclonal Antibody is a Rabbit antibody targeting KMT5A. Anti-KMT5A Polyclonal Antibody can be used in WB,FCM. |
| Ig Type | IgG |
| Reactivity | Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Sample: 293T (Human) Cell Lysate at 30 μg Primary: Anti-KMT5A (TMAB-08142) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 43 kD 2. Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice) Primary Antibody (green line): Rabbit Anti-KMT5A antibody (TMAB-08142), Dilution: 1 μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 μg /test. |
| Application | |
| Recommended Dose | WB: 1:500-2000; FCM: 1μg/Test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Chromosome. Specifically localizes to mitotic chromosomes. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. |
| Immunogen | KLH conjugated synthetic peptide: human KMT5A |
| Antigen Species | Human |
| Gene Name | KMT5A |
| Gene ID | |
| Protein Name | N-lysine methyltransferase KMT5A |
| Uniprot ID |
| Molecular Weight | Theoretical: 43 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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