Anti-KLHL24 Polyclonal Antibody is a Rabbit antibody targeting KLHL24. Anti-KLHL24 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Rabbit)

1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-KLHL24 Polyclonal Antibody, Unconjugated (TMAB-08109) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Small intestine (mouse) Lysate at 40 μg
Primary: Anti-KLHL24 (TMAB-08109) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 68 kD
3. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (KLHL24) Polyclonal Antibody, Unconjugated (TMAB-08109) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human KLHL24

Human

KLHL24

Kelch-like protein 24

Specifically reduces kainate receptor-mediated currents in hippocampal neurons, most probably by modulating channel properties.