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Anti-KLHL22 Antibody (5K493)
😃Good
Catalog No. TMAC-02332
Alias RGD1306288, Klhl 22, KLH22_HUMAN, Kelch-like protein 22, kelch-like family member 22, kelch-like 22, KELCHL, 2610318I18Rik
Anti-KLHL22 Antibody (5K493) is a Mouse antibody targeting KLHL22. Anti-KLHL22 Antibody (5K493) can be used in WB,ICC.
Anti-KLHL22 Antibody (5K493)
Anti-KLHL22 Antibody (5K493)
😃Good
Catalog No. TMAC-02332Alias RGD1306288, Klhl 22, KLH22_HUMAN, Kelch-like protein 22, kelch-like family member 22, kelch-like 22, KELCHL, 2610318I18Rik
Anti-KLHL22 Antibody (5K493) is a Mouse antibody targeting KLHL22. Anti-KLHL22 Antibody (5K493) can be used in WB,ICC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $297 | 7-10 days | |
| 100 μL | $427 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-KLHL22 Antibody (5K493) is a Mouse antibody targeting KLHL22. Anti-KLHL22 Antibody (5K493) can be used in WB,ICC. |
| Synonyms | RGD1306288, Klhl 22, KLH22_HUMAN, Kelch-like protein 22, kelch-like family member 22, kelch-like 22, KELCHL, 2610318I18Rik |
| Clone | 5K493 |
| Reactivity | Human,Mouse |
| Verified Activity | 1. Western blot analysis of KLHL22 on mouse brain tissue lysate using anti-KLHL22 antibody at 1/1,000 dilution. 2. ICC staining KLHL22 (green) and actin filaments (red) in U251 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| Application | |
| Recommended Dose | WB: 1:500; ICC: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide. |
| Research Background | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 72 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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