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Anti-KLF4 Polyclonal Antibody is a Rabbit antibody targeting KLF4. Anti-KLF4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $220 | 7-10 days | |
100 μL | $373 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-KLF4 Polyclonal Antibody is a Rabbit antibody targeting KLF4. Anti-KLF4 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-KLF4 Polyclonal Antibody, Unconjugated (TMAB-01026) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KLF4) Polyclonal Antibody, Unconjugated (TMAB-01026) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 3. Blank control: MCF7 (blue). Primary Antibody: Rabbit Anti-KLF4 AF647 Conjugated antibody (TMAB-01026-AF647), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647 (orange),used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (TMAB-01026-AF647, 1 μg/1x10^6 cells) for 30 min on ice. 4. Sample: 293T (human) cell Lysate at 40 μg Primary: Anti-KLF4 (TMAB-01026) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 60 kDa 5. Blank control: K562. Primary Antibody (green line): Rabbit Anti-KLF4 antibody (TMAB-01026) Dilution: 1 μg/10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 1 μg/test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 6. Sample: Hela Cell (Human) lysate at 30 μg Primary: Anti-KLF4 (TMAB-01026) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa 7. Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Human A431 cell lysates Lane 3: Human LOVO cell lysates Lane 4: Human HeLa cell lysates Lane 5: Human MCF-7 cell lysates Lane 6: Human A549 cell lysates Primary: Anti-KLF4 (TMAB-01026) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 50 kDa |
Application | WB,IHC-P,IHC-Fr,ICC/IF,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:100; IF: 1:50-200; FCM: 1ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor. |
Immunogen | KLH conjugated synthetic peptide: human KLF4 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Transcription factors,Tumor suppressors,Endothelial Cell Markers,Transcription factors,Surface molecules,Cell Cycle Inhibitors,Cell Differentiation,Krueppel like factor,Krueppel like factor,Other factors,Transcription Factors,Intracellular |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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