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Anti-KLF4 Antibody (8B968) is a Rabbit antibody targeting KLF4. Anti-KLF4 Antibody (8B968) can be used in WB,ICC/IF,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $297 | 7-10 days | |
100 μL | $498 | 7-10 days |
Description | Anti-KLF4 Antibody (8B968) is a Rabbit antibody targeting KLF4. Anti-KLF4 Antibody (8B968) can be used in WB,ICC/IF,IHC. |
Synonyms | Krueppel-like factor 4, KLF 4, Gut-enriched krueppel-like factor, GKLF, EZF, Epithelial zinc finger protein EZF |
Ig Type | IgG |
Clone | 8B968 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Western blot analysis of KLF4 on different lysates using anti-KLF4 antibody at 1/1,000 dilution. Positive control: Lane 1: 293, Lane 2: NCCIT. 2. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-KLF4 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-KLF4 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-KLF4 antibody. Counter stained with hematoxylin. 5. Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-KLF4 antibody. Counter stained with hematoxylin. 6. Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-KLF4 antibody. Counter stained with hematoxylin. 7. ICC staining KLF4 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 8. ICC staining KLF4 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 9. ICC staining KLF4 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Application | |
Recommended Dose | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | Recombinant Antibody |
Purification | ProA affinity purified |
Appearance | Liquid |
Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
Research Background | The Kruppel-type zinc finger transcription factors comprise a conserved family of DNA binding proteins that are important in developmental regulation. The Kruppel zinc finger transcription factor was initially identified in Drosophila as a segmentation gene. Kruppel-like factors that have been characterized in mammals include EKLF, LKLF and GKLF (6-8). EKLF is expressed principally in erythroid tissues, and LKLF expression is limited to the lung. GKLF is found predominantly in gut and has been shown to be expressed during growth arres. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein |
Uniprot ID |
Molecular Weight | Theoretical: 55 kDa. |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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