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Anti-KLF2 Polyclonal Antibody

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Catalog No. TMAB-01025

Anti-KLF2 Polyclonal Antibody is a Rabbit antibody targeting KLF2. Anti-KLF2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-KLF2 Polyclonal Antibody

Anti-KLF2 Polyclonal Antibody

😃Good
Catalog No. TMAB-01025
Anti-KLF2 Polyclonal Antibody is a Rabbit antibody targeting KLF2. Anti-KLF2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-KLF2 Polyclonal Antibody is a Rabbit antibody targeting KLF2. Anti-KLF2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Blank control: A431. Primary Antibody (green line): Rabbit Anti-KLF2 antibody (TMAB-01025)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0); Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30 min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (TMAB-01025) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.
3. Tissue/cell: Mouse embryos;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-KLF2 Polyclonal Antibody, Unconjugated (TMAB-01025) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus.
4. Sample:
Lane1: Brain (Mouse) Lysate at 30 μg
Lane2: HepG2 Cell Lysate at 30 μg
Primary: Anti-KLF2 (TMAB-01025) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit Igg (bse-0295G-HRP) at 1: 5000 dilution;
Predicted band size:39 kDa
Observed band size:39 kDa
5. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (TMAB-01025) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:100-500; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundKruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human KLF2
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Angiogenic inhibitory factors,Endothelium,Krueppel like factor,Krueppel like factor
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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