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Anti-KIFC1 Polyclonal Antibody
Catalog No. TMAB-08060 Copy Product Info
🥰Excellent
Anti-KIFC1 Polyclonal Antibody is a Rabbit antibody targeting KIFC1. Anti-KIFC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-KIFC1 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-08060
Anti-KIFC1 Polyclonal Antibody is a Rabbit antibody targeting KIFC1. Anti-KIFC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-KIFC1 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $373 | 7-10 days | 7-10 days | |
| 200 μL | $528 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
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| Description | Anti-KIFC1 Polyclonal Antibody is a Rabbit antibody targeting KIFC1. Anti-KIFC1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Pig,Cow,Horse) |
| Verified Activity | 1. Sample: Raji Cell (Human) Lysate at 40 μg Primary: Anti-KIFC1 (TMAB-08060) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 74 kD Observed band size: 74 kD 2. Sample: A549 Cell (Human) Lysate at 40 μg Primary: Anti-KIFC1 (TMAB-08060) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilution Predicted band size: 74 kD Observed band size: 74 kD 3. Paraformaldehyde-fixed, paraffin embedded (Human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIFC1) Polyclonal Antibody, Unconjugated (TMAB-08060) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (KIFC1) Polyclonal Antibody, Unconjugated (TMAB-08060) at 1: 200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Cytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | The kinesins constitute a large family of microtubule-dependent motor proteins, which are responsible for the distribution of numerous organelles, vesicles and macromolecular complexes throughout the cell. Individual kinesin members play crucial roles in cell division, intracellular transport and membrane trafficking events including endocytosis and transcytosis. KIFC1 is a 673 amino acid protein that belongs to the kinesin-like family of proteins. KIFC1 localizes to the nucleus and contains a C-terminal kinesin-motor domain. Functioning as a minus-end directed microtubule-dependent motor, KIFC1 works together with NuMA and cytoplasmic Dynein to organize microtubule minus ends at spindle poles. HeLa cells deficient in KIFC1 exhibit multipolar mitotic spindles, suggesting that KIFC1 is essential for bipolar spindle formation. |
| Immunogen | KLH conjugated synthetic peptide: human KIFC1 |
| Antigen Species | Human |
| Gene Name | KIFC1 |
| Gene ID | |
| Protein Name | Kinesin-like protein KIFC1 |
| Uniprot ID | |
| Function | Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. |
| Molecular Weight | Theoretical: 74 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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