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Anti-KIF5A/NKHC1 Polyclonal Antibody
Catalog No. TMAB-08050 Copy Product Info
🥰Excellent
Anti-KIF5A/NKHC1 Polyclonal Antibody is a Rabbit antibody targeting KIF5A/NKHC1. Anti-KIF5A/NKHC1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-KIF5A/NKHC1 Polyclonal Antibody
Copy Product Info🥰Excellent
Catalog No. TMAB-08050
Anti-KIF5A/NKHC1 Polyclonal Antibody is a Rabbit antibody targeting KIF5A/NKHC1. Anti-KIF5A/NKHC1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-KIF5A/NKHC1 Polyclonal Antibody
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $222 | 7-10 days | 7-10 days | |
| 100 μL | $371 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
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| Description | Anti-KIF5A/NKHC1 Polyclonal Antibody is a Rabbit antibody targeting KIF5A/NKHC1. Anti-KIF5A/NKHC1 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Mouse,Rat (predicted:Human,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIF5A/NKHC1) Polyclonal Antibody, Unconjugated (TMAB-08050) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (KIF5A/NKHC1) Polyclonal Antibody, Unconjugated (TMAB-08050) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Concentrated in the cell body of the neurons, particularly in the perinuclear region. |
| Tissue Specificity | Distributed throughout the CNS but is highly enriched in subsets of neurons. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | NKHC1 is a neuronal-specific component of a multi-subunit “molecular motor” complex that mediates intracellular organelle transport. Mutations in the gene encoding NKHC1 cause autosomal dominant spastic paraplegia 10. NKHC1 has a pan-neuronal distribution in the nervous system. Rat tissue extracts by immunoblot of NKHC1 can produce a doublet only in brain and sciatic nerve tissue. NKHC1 is distributed throughout the central nervous system and is enriched in subsets of neurons. Within cultured hippocampal neurons, NKHC1 is concentrated in the perinuclear region of the cell body. Kinesin superfamily proteins like NKHC1 are the molecular motors conveying cargos along microtubules. |
| Immunogen | KLH conjugated synthetic peptide: human KIF5A/NKHC1 |
| Antigen Species | Human |
| Gene Name | KIF5A |
| Gene ID | |
| Protein Name | Kinesin heavy chain isoform 5A |
| Uniprot ID | |
| Function | Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). |
| Molecular Weight | Theoretical: 113 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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