Anti-KIF4A Antibody (8C211) is a Rabbit antibody targeting KIF4A. Anti-KIF4A Antibody (8C211) can be used in WB,FCM,ICC/IF.

IgG

8C211

Human,Mouse

1. 25 μg total protein per lane of various lysates (see on figure) probed with KIF4A monoclonal antibody, unconjugated (TMAB-08049) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (KIF4A) monoclonal Antibody, unconjugated (TMAB-08049) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

WB: 1:500-2000; FCM: 1:50-100; ICC/IF: 1:50-200

Monoclonal

A synthesized peptide: human Chromokinesin A

Human

KIF4A

Chromosome-associated kinesin KIF4A

Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.