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Anti-KIF3A Antibody (5I460) is a Rabbit antibody targeting KIF3A. Anti-KIF3A Antibody (5I460) can be used in WB,IP,FCM.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 25 μL | $150 | 7-10 days | 7-10 days | |
| 50 μL | $261 | 7-10 days | 7-10 days | |
| 100 μL | $471 | 7-10 days | 7-10 days |
| Description | Anti-KIF3A Antibody (5I460) is a Rabbit antibody targeting KIF3A. Anti-KIF3A Antibody (5I460) can be used in WB,IP,FCM. |
| Ig Type | IgG |
| Clone | 5I460 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of SH-SY5Y cell lysate. Using KIF3A (TMAB-08047) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min. 2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-KIF3A antibody (TMAB-08047, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IP: 1:20-50; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytoskeleton (Probable). Cell projection, cilium (By similarity). |
| Construction | Recombinant Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| Research Background | Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole. |
| Immunogen | A synthesized peptide: human KIF3A |
| Antigen Species | Human |
| Gene Name | KIF3A |
| Gene ID | |
| Protein Name | Kinesin-like protein KIF3A |
| Uniprot ID | |
| Function | Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. |
| Molecular Weight | Theoretical: 80 kDa. Actual: 80 kDa. |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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