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Anti-KIF3A Antibody (5I460)

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Catalog No. TMAB-08047

Anti-KIF3A Antibody (5I460) is a Rabbit antibody targeting KIF3A. Anti-KIF3A Antibody (5I460) can be used in WB,IP,FCM.

Anti-KIF3A Antibody (5I460)

Anti-KIF3A Antibody (5I460)

Copy Product Info
😃Good
Catalog No. TMAB-08047
Anti-KIF3A Antibody (5I460) is a Rabbit antibody targeting KIF3A. Anti-KIF3A Antibody (5I460) can be used in WB,IP,FCM.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
25 μL$1507-10 days7-10 days
50 μL$2617-10 days7-10 days
100 μL$4717-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-KIF3A Antibody (5I460) is a Rabbit antibody targeting KIF3A. Anti-KIF3A Antibody (5I460) can be used in WB,IP,FCM.
Ig Type
IgG
Clone
5I460
Reactivity
Human,Mouse,Rat
Verified Activity
1. Western blot analysis of SH-SY5Y cell lysate. Using KIF3A (TMAB-08047) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-KIF3A antibody (TMAB-08047, 1:100); Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
Application
Recommended Dose
WB: 1:1000-2000; IP: 1:20-50; FCM: 1:50-100
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm, cytoskeleton (Probable). Cell projection, cilium (By similarity).
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundMicrotubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitment of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole.
Antigen Details
Immunogen
A synthesized peptide: human KIF3A
Antigen Species
Human
Gene Name
KIF3A
Gene ID
Protein Name
Kinesin-like protein KIF3A
Uniprot ID
Function
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
Chemical Properties
Molecular WeightTheoretical: 80 kDa. Actual: 80 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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