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Anti-KIF11 Polyclonal Antibody

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Catalog No. TMAB-08029

Anti-KIF11 Polyclonal Antibody is a Rabbit antibody targeting KIF11. Anti-KIF11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

Anti-KIF11 Polyclonal Antibody

Anti-KIF11 Polyclonal Antibody

Copy Product Info
😃Good
Catalog No. TMAB-08029
Anti-KIF11 Polyclonal Antibody is a Rabbit antibody targeting KIF11. Anti-KIF11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
50 μL$2217-10 days7-10 days
100 μL$3727-10 days7-10 days
200 μL$5277-10 days7-10 days
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction

Bioactivity
Description
Anti-KIF11 Polyclonal Antibody is a Rabbit antibody targeting KIF11. Anti-KIF11 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit)
Verified Activity
1. Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-KIF11 Polyclonal Antibody, Unconjugated (TMAB-08029) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample: Testis (Mouse) Lysate at 40 μg
Primary: Anti-KIF11 (TMAB-08029) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Cytoplasm, cytoskeleton, spindle pole.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Research BackgroundEg5 is a motor protein that belongs to the kinesin-like family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human KIF11/Eg5
Antigen Species
Human
Gene Name
KIF11
Gene ID
Protein Name
Kinesin-like protein KIF11
Uniprot ID
Function
Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Chemical Properties
Molecular WeightTheoretical: 120 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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