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Anti-Ki67/MKI67 Antibody (6Q325)
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Catalog No. TMAY-02523
Alias PPP1R105, MIB-1, MIB-, marker of proliferation Ki-67, KIA
Anti-Ki67/MKI67 Antibody (6Q325) is a Mouse antibody targeting Ki67/MKI67. Anti-Ki67/MKI67 Antibody (6Q325) can be used in WB,ICC/IF.
Anti-Ki67/MKI67 Antibody (6Q325)
Anti-Ki67/MKI67 Antibody (6Q325)
😃Good
Catalog No. TMAY-02523Alias PPP1R105, MIB-1, MIB-, marker of proliferation Ki-67, KIA
Anti-Ki67/MKI67 Antibody (6Q325) is a Mouse antibody targeting Ki67/MKI67. Anti-Ki67/MKI67 Antibody (6Q325) can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ICC/IF Reactivity: Human |
| Alias | PPP1R105, MIB-1, MIB-, marker of proliferation Ki-67, KIA |
| Ig Type | Mouse IgG1 |
| Clone | 6Q325 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human MKI67 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human MKI67 monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus. 2. Anti-MKI67 mouse monoclonal antibody at 1:500 dilution. -Lane A: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:358 kDa. -Observed band size:358 kDa |
| Application | WB,ICC/IF |
| Recommended Dose | WB: 1:500-1:1000; ICC-IF: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MKI67 (rh MKI67; TMPY-03105; P46013-2; Met1-Pro120). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | MKI67 contains 1 FHA domain and plays a key role in cell proliferation. During interphase, the MKI67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. MKI67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells. MKI67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of MKI67-positive tumor cells is often correlated with the clinical course of cancer. It is also associated with ribosomal RNA transcription. Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis. The MKI67 protein is a nuclear and nucleolar protein, which is tightly associated with somatic cell proliferation. Antibodies raised against the human MKI67 protein paved the way for the immunohistological assessment of cell proliferation, particularly useful in numerous studies on the prognostic value of cell growth in clinical samples of human neoplasms. MKI67 protein expression is an absolute requirement for progression through the cell-division cycle. Recently, MKI67 is proved to be an independent prognostic factor for disease-free survival (HR 1.5-1.72) in multivariate analyses studies using samples from randomized clinical trials with secondary central analysis of the biomarker. MKI67 was not found to be predictive for long-term follow-up after chemotherapy. Nevertheless, high KI-67 was found to be associated with immediate pathological complete response in the neoadjuvant setting, with an LOE of II-B. MKI67 could be considered as a prognostic biomarker for therapeutic decision. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human MKI67 protein (TMPY-03105) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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