Anti-Ki-67 Antibody (6E719) is a Mouse antibody targeting Ki-67. Anti-Ki-67 Antibody (6E719) can be used in IHC-P,IHC-Fr,IF,FCM,ICC/IF.

IgG

6E719

Human

1. The Hela (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Mouse Anti-Ki-67 antibody cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (Ki-67) monoclonal Antibody, unconjugated 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
2. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (TMAB-07997) at 1: 1000 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Serous ovarian cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (TMAB-07997) at 1: 1000 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (TMAB-07997) at 1: 1000 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (TMAB-07997) at 1: 1000 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
6. (Negative control) Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Ki-67 Monoclonal Antibody, Unconjugated (TMAB-07997) at 1: 1000 overnight at 4°C, followed by conjugation to the HRP and DAB staining.

IHC-P: 1:1000-5000; IHC-Fr: 1:1000-5000; IF: 1:1000-5000; FCM: 1μg/Test; ICC/IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human Ki67

Human

MKI67

Proliferation marker protein Ki-67

Thought to be required for maintaining cell proliferation.