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Anti-KDM1B Antibody (3V868)
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Catalog No. TMAC-00221
Alias Lysine-specific histone demethylase 2, Lysine-specific histone demethylase 1B, LSD2, Flavin-containing amine oxidase domain-containing protein 1, C6orf193, AOF1
Anti-KDM1B Antibody (3V868) is a Mouse antibody targeting KDM1B. Anti-KDM1B Antibody (3V868) can be used in WB,ICC,IHC,FCM.
Anti-KDM1B Antibody (3V868)
Anti-KDM1B Antibody (3V868)
😃Good
Catalog No. TMAC-00221Alias Lysine-specific histone demethylase 2, Lysine-specific histone demethylase 1B, LSD2, Flavin-containing amine oxidase domain-containing protein 1, C6orf193, AOF1
Anti-KDM1B Antibody (3V868) is a Mouse antibody targeting KDM1B. Anti-KDM1B Antibody (3V868) can be used in WB,ICC,IHC,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $296 | 7-10 days | |
| 100 μL | $428 | 7-10 days |
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| Description | Anti-KDM1B Antibody (3V868) is a Mouse antibody targeting KDM1B. Anti-KDM1B Antibody (3V868) can be used in WB,ICC,IHC,FCM. |
| Synonyms | Lysine-specific histone demethylase 2, Lysine-specific histone demethylase 1B, LSD2, Flavin-containing amine oxidase domain-containing protein 1, C6orf193, AOF1 |
| Reactivity | Human |
| Verified Activity | 1. Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1,000 dilution. 2. Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1,000 dilution. 3. Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin. 5. ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC: 1:50-200; ICC: 1:50-200; FCM: 1:100-200 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | AOF1 (amine-oxidase flavin-containing domain 1), whose alternative names include KDM1B (lysine (K)-specific demethylase 1B) or LSD2 (lysine-specific histone demethylase 2), is an 823 amino acid nuclear protein belonging to the flavin monoamine oxidase family. As a histone demethylase, AOF1 specifically demethylates Lys4 of histone H3, a marker for epigenetic transcriptional activation, by interacting with a long stretch of the H3 N-terminal tail of mono- and dimethylated Lys4. Existing as three alternatively spliced isoforms, AOF1 is required for de novo DNA methylation of some imprinted genes in oocytes and contains a single SWIRM domain that is implicated in chromatin regulation. AOF1 contains one CW-type zinc finger and is encoded by a gene located on human chromosome 6p22.3. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 92 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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