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Anti-KDEL Antibody (2I425)

Catalog No. TMAB-07969 Copy Product Info
🥰Excellent
Anti-KDEL Antibody (2I425) is a Rabbit antibody targeting KDEL. Anti-KDEL Antibody (2I425) can be used in FCM, ICC/IF, WB.

Anti-KDEL Antibody (2I425)

Copy Product Info
🥰Excellent
Catalog No. TMAB-07969

Anti-KDEL Antibody (2I425) is a Rabbit antibody targeting KDEL. Anti-KDEL Antibody (2I425) can be used in FCM, ICC/IF, WB.

Anti-KDEL Antibody
(2I425)
Pack SizePriceUSA StockGlobal StockQuantity
25 μL$1507-10 days7-10 days
50 μL$2647-10 days7-10 days
100 μL$4737-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-KDEL Antibody (2I425) is a Rabbit antibody targeting KDEL. Anti-KDEL Antibody (2I425) can be used in FCM, ICC/IF, WB.
Ig Type
IgG
Clone
2I425
Reactivity
Human,Mouse,Rat
Verified Activity
1. 25 ug total protein per lane of various lysates (see on figure) probed with KDEL monoclonal antibody, unconjugated (TMAB-07969) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. 4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (KDEL) monoclonal Antibody, unconjugated (TMAB-07969) 1: 100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Application
Recommended Dose
FCM=1:50-100; ICC/IF=1:50-200; WB=1:500-2000
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum lumen.
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundThe C-terminal sequence motif K-D-E-L that is present on endoplasmic reticulum resident proteins and that mediates their recycling from the Golgi back to the endoplasmic reticulum.
Antigen Details
Immunogen
A synthesized peptide: KDEL
Antigen Species
Human
Gene Name
POGLUT2
Gene ID
Protein Name
Protein O-glucosyltransferase 2
Uniprot ID
Chemical Properties
Molecular WeightTheoretical: 25 kDa. Actual: 78 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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