Anti-KCTD7 Polyclonal Antibody is a Rabbit antibody targeting KCTD7. Anti-KCTD7 Polyclonal Antibody can be used in FCM.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-KCTD7 antibody (TMAB-07964)
Dilution: 1 μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg /test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human KCTD7

Human

KCTD7

BTB/POZ domain-containing protein KCTD7

The KCTD gene family, including KCTD7, encode predicted proteins that contain N terminal domain that is homologous to the T1 domain in voltage gated potassium channels. KCTD7 displays a primary sequence and hydropathy profile indicating intracytoplasmic localization. There are two named isoforms.