Anti-KCNH1 Polyclonal Antibody is a Rabbit antibody targeting KCNH1. Anti-KCNH1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Rabbit)

1. Blank control: Hela (blue). Primary Antibody: Rabbit Anti-KCNH1 antibody (TMAB-07931), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1: 200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA+10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of 000 events was performed.
2. Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human HeLa cell lysates
Primary: Anti-KCNH1 (TMAB-07931) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kDa
Observed band size: 108, 110 kDa

WB: 1:500-2000; FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human KCNH1

Human

KCNH1

Potassium voltage-gated channel subfamily H member 1

Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts.