Anti-KCNA7 Polyclonal Antibody is a Rabbit antibody targeting KCNA7. Anti-KCNA7 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Chicken,Dog,Pig,Rabbit)

1. Sample: Muscle (Mouse) Lysate at 40 μg
Primary: Anti-KCNA7 (TMAB-07923) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-KCNA7 Polyclonal Antibody, Unconjugated (TMAB-07923) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
3. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Mouse Tongue tissue lysates
Primary: Anti-KCNA7 (TMAB-07923) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 51 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human KCNA7

Human

KCNA7

Potassium voltage-gated channel subfamily A member 7

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient (By similarity).