Anti-JUNB Antibody (8B522) is a Rabbit antibody targeting JUNB. Anti-JUNB Antibody (8B522) can be used in IHC-P,IHC-Fr,ICC/IF,IF,WB,FCM.

IgG

8B522

Human,Mouse

1. 25 ug total protein per lane of various lysates (see on figure) probed with JUNB monoclonal antibody, unconjugated (TMAB-07890) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with JUNB Monoclonal Antibody, Unconjugated (TMAB-07890) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with JUNB Monoclonal Antibody, Unconjugated (TMAB-07890) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Mouse Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with JUNB Monoclonal Antibody, Unconjugated (TMAB-07890) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with JUNB Monoclonal Antibody, Unconjugated (TMAB-07890) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with JUNB Monoclonal Antibody, Unconjugated (TMAB-07890) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with JUNB Monoclonal Antibody, Unconjugated (TMAB-07890) at 1:200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L-HRP and DAB staining.
8. 4% Paraformaldehyde-fixed A431 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (JunB) monoclonal Antibody, unconjugated (TMAB-07890) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200; WB: 1:500-2000; FCM: 1:50-100

Monoclonal

JUNB

Transcription factor jun-B

Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.