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Anti-JNK2 Antibody (2W411)
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Catalog No. TMAY-01115
Alias SAPK1a, SAPK, PRKM9, p54aSAPK, p54a, mitogen-activated protein kinase 9, JNK-55, JNK2β, JNK2α, JNK2BETA, JNK2B, JNK2ALPHA, JNK2A, JNK2
Anti-JNK2 Antibody (2W411) is a Mouse antibody targeting JNK2. Anti-JNK2 Antibody (2W411) can be used in FCM,ICC/IF.
Anti-JNK2 Antibody (2W411)
Anti-JNK2 Antibody (2W411)
😃Good
Catalog No. TMAY-01115Alias SAPK1a, SAPK, PRKM9, p54aSAPK, p54a, mitogen-activated protein kinase 9, JNK-55, JNK2β, JNK2α, JNK2BETA, JNK2B, JNK2ALPHA, JNK2A, JNK2
Anti-JNK2 Antibody (2W411) is a Mouse antibody targeting JNK2. Anti-JNK2 Antibody (2W411) can be used in FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-JNK2 Antibody (2W411) is a Mouse antibody targeting JNK2. Anti-JNK2 Antibody (2W411) can be used in FCM,ICC/IF. |
| Alias | SAPK1a, SAPK, PRKM9, p54aSAPK, p54a, mitogen-activated protein kinase 9, JNK-55, JNK2β, JNK2α, JNK2BETA, JNK2B, JNK2ALPHA, JNK2A, JNK2 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 2W411 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human MAPK9 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus. 2. Flow cytometric analysis of Human MAPK9 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human MAPK9, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | FCM,ICC/IF |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JNK2 / MAPK9 (rh JNK2 / MAPK9; TMPY-04550; NP_002743.3; Met1-Arg424). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Mitogen-activated protein kinase 9 (MAPK9), also well known as c-Jun N-terminal kinase (JNK2), is a member of the MAP kinase subfamily belonging to the protein kinase superfamily. MAPK9 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating some transcription factors, such as c-Jun and ATF2. The crystal structure of human JNK2 complexed with an indazole inhibitor by applying a high-throughput protein engineering and surface-site mutagenesis approach. A novel conformation of the activation loop is observed, which is not compatible with its phosphorylation by upstream kinases. This activation inhibitory conformation of JNK2 is stabilized by the MAP kinase insert that interacts with the activation loop in an induced-fit manner. It suggests that the MAP kinase insert of JNK2 plays a role in the regulation of JNK2 activation, possibly by interacting with intracellular binding partners. JNK2 deficiency leads to reduced c-Jun degradation, thereby augmenting c-Jun levels and cellular proliferation, and suggests that JNK2 is a negative regulator of cellular proliferation in multiple cell types. JNK2 prevents replicative stress by coordinating cell cycle progression and DNA damage repair mechanisms. JNK2 blocks the ubiquitination of tumor suppressor p53, and thus increases the stability of p53 in nonstressed cells. JNK2 negatively regulates antigen-specific CD8+ T cell expansion and effector function, and thus selectively blocking JNK2 in CD8+ T cells may potentially enhance the anti-tumor immune response. Lack of JNK2 expression was associated with higher tumor aneuploidy and reduced DNA damage response. Additionally, the JNK2 protein could be a novel therapeutic target in dry eye disease and may provide a novel target for the prevention of vascular disease and atherosclerosis. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human JNK2 / MAPK9 protein (TMPY-04550) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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