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Anti-JAM-A Antibody (5E755)
😃Good
Catalog No. TMAY-00440
Alias F11 receptor
Anti-JAM-A Antibody (5E755) is a Mouse antibody targeting JAM-A. Anti-JAM-A Antibody (5E755) can be used in FCM,ICC/IF.
Anti-JAM-A Antibody (5E755)
Anti-JAM-A Antibody (5E755)
😃Good
Catalog No. TMAY-00440Alias F11 receptor
Anti-JAM-A Antibody (5E755) is a Mouse antibody targeting JAM-A. Anti-JAM-A Antibody (5E755) can be used in FCM,ICC/IF.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $199 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-JAM-A Antibody (5E755) is a Mouse antibody targeting JAM-A. Anti-JAM-A Antibody (5E755) can be used in FCM,ICC/IF. |
| Synonyms | F11 receptor |
| Ig Type | Mouse IgG1 |
| Clone | 5E755 |
| Reactivity | Human |
| Specificity | Human JAM-A/F11 Receptor |
| Verified Activity | 1. Immunofluorescence staining of Human JAM-A in A431 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human JAM-A monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated (left panel, captured by laser confocal scanning microscope) and Alexa Fluor®594-conjugated (right panel, captured by fluorescence microscope) Goat Anti-mouse IgG secondary antibody, countstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of human JAM-A(CD321) expression on SKBR3 cells. Cells were stained with purified anti-Human JAM-A(CD321), then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells. |
| Application | |
| Recommended Dose | ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human JAM-A / F11R / CD321 (rh JAM-A / F11R / CD321; TMPY-01035; NP_058642.1; Met1-Ala242). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer. |
| Conjucates | Unconjugated |
| Others Formats | APC/FITC/PE |
| Antibody Types Available | 4 |
| Immunogen | Recombinant Protein: Human JAM-A / F11R / CD321 protein (TMPY-01035) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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