Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,ICC/IF,IF

Reactivity: Human (predicted:Mouse,Rat)

IgG

Human (predicted:Mouse,Rat)

1. Tissue/cell: human stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Jagged1 Polyclonal Antibody, Unconjugated (TMAB-01001) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: Human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Jagged1 Polyclonal Antibody, Unconjugated (TMAB-01001) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Jagged1) polyclonal Antibody, Unconjugated (TMAB-01001) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
4. Sample:
Lane 1: Recombinant human CD339 protein, N-His
Primary: Anti-Jagged1 (TMAB-01001) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kDa
Observed band size: 22 kDa

WB,IHC-P,IHC-Fr,ICC/IF,IF

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human CD339

Human

182

Inhibitors,Neurogenesis,Notch Pathway,Surface Molecules,Surface Molecules