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Anti-ITCH/AIP4 Antibody (6J974)

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Catalog No. TMAY-01671
Alias NAPP1, ITCH, AIP4, AIP 4, AIF4, ADMFD

Anti-ITCH/AIP4 Antibody (6J974) is a Mouse antibody targeting ITCH/AIP4. Anti-ITCH/AIP4 Antibody (6J974) can be used in FCM,ICC/IF.

Anti-ITCH/AIP4 Antibody (6J974)

Anti-ITCH/AIP4 Antibody (6J974)

😃Good
Catalog No. TMAY-01671Alias NAPP1, ITCH, AIP4, AIP 4, AIF4, ADMFD
Anti-ITCH/AIP4 Antibody (6J974) is a Mouse antibody targeting ITCH/AIP4. Anti-ITCH/AIP4 Antibody (6J974) can be used in FCM,ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ITCH/AIP4 Antibody (6J974) is a Mouse antibody targeting ITCH/AIP4. Anti-ITCH/AIP4 Antibody (6J974) can be used in FCM,ICC/IF.
AliasNAPP1, ITCH, AIP4, AIP 4, AIF4, ADMFD
Ig Type
Monoclonal Mouse IgG1
Clone
6J974
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITCH monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

2. Flow cytometric analysis of Human ITCH expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human ITCH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
FCM,ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITCH / AIP4 (rh ITCH / AIP4; NP_113671.3; Arg526-Glu903). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundE3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1, and ITCH, is a cell membrane protein that contains one C2 domain, one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains four WW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1, and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of the syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features, and autoimmune inflammatory cell infiltration of the lungs, liver, and gut.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC
Antibody Types Available
2
Antigen Details
Immunogen
Recombinant Human ITCH / AIP4 protein
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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