Anti-ISX/Intestine specific homeobox Polyclonal Antibody is a Rabbit antibody targeting ISX/Intestine specific homeobox. Anti-ISX/Intestine specific homeobox Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Cow,Horse,Sheep)

1. Sample:
small intestine (Mouse) Lysate at 40 μg
large intestine (Mouse) Lysate at 40 μg
Primary: Anti-ISX/Intestine specific homeobox (TMAB-07827) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
2. Sample:
Lane 1: Lovo (Human) Cell Lysate at 30 μg
Lane 2: SW480 (Human) Cell Lysate at 30 μg
Primary: Anti-ISX/Intestine specific homeobox (TMAB-07827) at 1:500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
3. Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated (TMAB-07827) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human ISX/Intestine specific homeobox

Human

ISX

Intestine-specific homeobox

Transcription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCMO1 expression in the intestine.