Anti-IRS3 Polyclonal Antibody is a Rabbit antibody targeting IRS3. Anti-IRS3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Rat,Pig (predicted:Mouse)

1. Tissue/cell: human pneumonia tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IRS-3 Polyclonal Antibody, Unconjugated (TMAB-07816) 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Sample:
Lane 1: Adipose (Rat) Lysate at 40 μg
Primary: Anti-IRS3 (TMAB-07816) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
3. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IRS3) Polyclonal Antibody, Unconjugated (TMAB-07816) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: mouse IRS-3

Mouse

Irs3

Insulin receptor substrate-3