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Anti-IRF6 Antibody (5N5) is a Mouse antibody targeting IRF6. Anti-IRF6 Antibody (5N5) can be used in ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $199 | 7-10 days |
Description | Anti-IRF6 Antibody (5N5) is a Mouse antibody targeting IRF6. Anti-IRF6 Antibody (5N5) can be used in ICC/IF. |
Alias | VWS1, VWS, PPS1, PPS, PIT, OFC6, LPS |
Ig Type | Monoclonal Mouse IgG3 |
Clone | 5N5 |
Reactivity | Human |
Verified Activity | Immunofluorescence staining of Human IRF6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human IRF6 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus. |
Application | ICC/IF |
Recommended Dose | ICC-IF: 1:20-1:100 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human IRF6. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | IRF6 (Interferon Regulatory Factor 6) is a Protein Coding gene. IRF6, a member of the IRF family of transcription factors, is required for the differentiation of skin, breast epithelium, and oral epithelium. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. IRF6, the gene that causes van der Woude syndrome (VWS), is a candidate gene for nonsyndromic cleft lip with or without cleft palate (NSCLP) because some studies have supported an association between NSCLP and single nucleotide polymorphisms (SNPs) in IRF6 in several populations. Interferon Regulatory Factor 6 (IRF6) gene variants are significant genetic contributors to orofacial clefts. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide corresponding to the C-terminus of the human IRF6 |
Antigen Species | Human |
Biology Area | Apoptosis Transcription Factors and Regulators |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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