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Anti-IRF6 Antibody (5N5)

😃Good
Catalog No. TMAY-00115
Alias VWS1, VWS, PPS1, PPS, PIT, OFC6, LPS

Anti-IRF6 Antibody (5N5) is a Mouse antibody targeting IRF6. Anti-IRF6 Antibody (5N5) can be used in ICC/IF.

Anti-IRF6 Antibody (5N5)

Anti-IRF6 Antibody (5N5)

😃Good
Catalog No. TMAY-00115Alias VWS1, VWS, PPS1, PPS, PIT, OFC6, LPS
Anti-IRF6 Antibody (5N5) is a Mouse antibody targeting IRF6. Anti-IRF6 Antibody (5N5) can be used in ICC/IF.
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100 μL$199 7-10 days
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Product Introduction

Bioactivity
Description
Anti-IRF6 Antibody (5N5) is a Mouse antibody targeting IRF6. Anti-IRF6 Antibody (5N5) can be used in ICC/IF.
AliasVWS1, VWS, PPS1, PPS, PIT, OFC6, LPS
Ig Type
Monoclonal Mouse IgG3
Clone
5N5
Reactivity
Human
Verified Activity
Immunofluorescence staining of Human IRF6 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human IRF6 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.
Application
ICC/IF
Recommended Dose
ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the C-terminus of the human IRF6. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundIRF6 (Interferon Regulatory Factor 6) is a Protein Coding gene. IRF6, a member of the IRF family of transcription factors, is required for the differentiation of skin, breast epithelium, and oral epithelium. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. IRF6, the gene that causes van der Woude syndrome (VWS), is a candidate gene for nonsyndromic cleft lip with or without cleft palate (NSCLP) because some studies have supported an association between NSCLP and single nucleotide polymorphisms (SNPs) in IRF6 in several populations. Interferon Regulatory Factor 6 (IRF6) gene variants are significant genetic contributors to orofacial clefts.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide corresponding to the C-terminus of the human IRF6
Antigen Species
Human
Biology Area
Apoptosis Transcription Factors and Regulators
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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