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Anti-IRF3 Polyclonal Antibody

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Catalog No. TMAB-00985
Alias IRF-3, Irf3, Interferon regulatory factor 3

Anti-IRF3 Polyclonal Antibody is a Rabbit antibody targeting IRF3. Anti-IRF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

Anti-IRF3 Polyclonal Antibody

Anti-IRF3 Polyclonal Antibody

😃Good
Catalog No. TMAB-00985Alias IRF-3, Irf3, Interferon regulatory factor 3
Anti-IRF3 Polyclonal Antibody is a Rabbit antibody targeting IRF3. Anti-IRF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2217-10 days
100 μL$3727-10 days
200 μL$5277-10 days
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Product Introduction

Bioactivity
Description
Anti-IRF3 Polyclonal Antibody is a Rabbit antibody targeting IRF3. Anti-IRF3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
AliasIRF-3, Irf3, Interferon regulatory factor 3
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse)
Verified Activity
1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IRF3 Polyclonal Antibody, Unconjugated (TMAB-00985) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-IRF3 Polyclonal Antibody, Unconjugated (TMAB-00985) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Heart (Mouse) Lysate at 40 μg
Heart (Rat) Lysate at 40 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-IRF3 (TMAB-00985) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
4. Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-IRF3 antibody (TMAB-00985)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
WB,IHC-P,IHC-Fr,IF,FCM
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human IRF3
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
SARS Coronavirus,TLR Signaling,Interferons,Other factors
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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