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Anti-IRF1 Antibody (2Q473)
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Catalog No. TMAC-02215
Alias IRF-1, IRF1, Interferon regulatory factor 1
Anti-IRF1 Antibody (2Q473) is a Rabbit antibody targeting IRF1. Anti-IRF1 Antibody (2Q473) can be used in WB,ICC/IF,IHC,IP,FCM.
Anti-IRF1 Antibody (2Q473)
Anti-IRF1 Antibody (2Q473)
😃Good
Catalog No. TMAC-02215Alias IRF-1, IRF1, Interferon regulatory factor 1
Anti-IRF1 Antibody (2Q473) is a Rabbit antibody targeting IRF1. Anti-IRF1 Antibody (2Q473) can be used in WB,ICC/IF,IHC,IP,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $298 | 7-10 days | |
| 100 μL | $498 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-IRF1 Antibody (2Q473) is a Rabbit antibody targeting IRF1. Anti-IRF1 Antibody (2Q473) can be used in WB,ICC/IF,IHC,IP,FCM. |
| Synonyms | IRF-1, IRF1, Interferon regulatory factor 1 |
| Ig Type | IgG |
| Clone | 2Q473 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Western blot analysis of IRF1 on different lysates using anti-IRF1 antibody at 1/1,000 dilution. Positive control: Lane 1: PC-12, Lane 2: Jurkat. 2. Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IRF1 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IRF1 antibody. Counter stained with hematoxylin. 4. ICC staining IRF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining IRF1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. Flow cytometric analysis of Jurkat cells with IRF1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
| Application | |
| Recommended Dose | WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | Recombinant Antibody |
| Purification | ProA affinity purified |
| Appearance | Liquid |
| Formulation | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Research Background | Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon- and ??) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon- and interferon-?? genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3 p48 (a component of the ISGF-3 complex) and IFN consensus sequence-binding protein (ICSBP). |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein |
| Uniprot ID |
| Molecular Weight | Theoretical: 48 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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