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Anti-IREB2 Polyclonal Antibody
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Catalog No. TMAB-00984
Alias OTTHUMP00000185022, IRP2AD, IRP-2, irp2, IRP2, Iron-responsive element-binding protein 2, iron regulatory protein 2, IRE-BP 2, IREB2, IREB 2, IRE BP2, FLJ23381, ACO3
Anti-IREB2 Polyclonal Antibody is a Rabbit antibody targeting IREB2. Anti-IREB2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-IREB2 Polyclonal Antibody
Anti-IREB2 Polyclonal Antibody
😃Good
Catalog No. TMAB-00984Alias OTTHUMP00000185022, IRP2AD, IRP-2, irp2, IRP2, Iron-responsive element-binding protein 2, iron regulatory protein 2, IRE-BP 2, IREB2, IREB 2, IRE BP2, FLJ23381, ACO3
Anti-IREB2 Polyclonal Antibody is a Rabbit antibody targeting IREB2. Anti-IREB2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-IREB2 Polyclonal Antibody is a Rabbit antibody targeting IREB2. Anti-IREB2 Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | OTTHUMP00000185022, IRP2AD, IRP-2, irp2, IRP2, Iron-responsive element-binding protein 2, iron regulatory protein 2, IRE-BP 2, IREB2, IREB 2, IRE BP2, FLJ23381, ACO3 |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Rabbit) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IREB2) Polyclonal Antibody, Unconjugated (TMAB-00984) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IREB2) Polyclonal Antibody, Unconjugated (TMAB-00984) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IREB2) Polyclonal Antibody, Unconjugated (TMAB-00984) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Iron metabolism is essential for sustaining mammalian homeostasis. Iron uptake and distribution is a highly regulated process in mammalian cells that is monitored by two iron sensing proteins: IRP-1 and -2 (iron regulatory protein-1 and -2), also known as iron responsive element-binding protein-1 and -2 (IRE-BP-1 and -2) or aconitase 1 and 2. IRP-1 and IRP-2 are important soluble regulatory factors that mediate iron uptake and storage in mammalian cells. They are capable of either repressing translation or enhancing mRNA stability by associating with stem-loop motifs known as iron-responsive elements (IREs). IRPs respond to stress mediators, iron concentration and signaling factors, including nitrogen monoxide, cytokines and hydrogen peroxide. |
| Immunogen | KLH conjugated synthetic peptide: human IREB2 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Blood,Hypoxia-Regulated,RNA Processing,Vitamins / minerals,Energy Metabolism,Nucleotide metabolism,Hypoxia,Energy Metabolism,Vitamins / Minerals |
| Molecular Weight | Theoretical: 105 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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