Anti-IRAK1BP1 Polyclonal Antibody is a Rabbit antibody targeting IRAK1BP1. Anti-IRAK1BP1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Rabbit)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01 M, pH 6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37℃ for 20 min;
Incubation: Anti-IRAK1BP1 Polyclonal Antibody, Unconjugated (TMAB-07788) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
2. Protein: MCF-7 (human) lyates at 40 μg;
Primary: Rabbit Anti-IRAK1BP1 (TMAB-07788) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit Igg (H+L) at 1: 10000;
Predicted band size: 29 kD
Observed band size:33 kD
3. Sample: Brain (Mouse) Lysate at 40 μg
Primary: Anti-IRAK1BP1 (TMAB-07788) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human IRAK1BP1

Human

IRAK1BP1

Interleukin-1 receptor-associated kinase 1-binding protein 1

Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. similarity). {ECO:0000250}.